This experiment is for advanced students.

How do you know if your brother is stealing your candy? Unwrap a wrapped hard candy that he likes a lot. Roll the candy around in the powdered food dye that matches the candy. (Push the powder into the candy so it “disappears”.) Re-wrap the candy. Set the candy in the place where it usually disappears from. Wait ten minutes after the candy disappears. Find your brother. He will be sporting a new color on his hands and mouth. Dye is hard to remove. It will have to be worn every day at school until it fades away as the skin cells slough off. The dye he now wears is in indicator that he has been taking your candy.

[am4show have='p9;p52;' guest_error='Guest error message' user_error='User error message' ] Did you guess that this lab is about indicators? A reagent is chemical compound that creates a reaction in another substance; the product of that chemical reaction is an indicator of the presence, absence, or concentration of another substance.

Your brother’s situation is not a chemical reaction, but a reaction will be observed in his looks and his mood.

We are going to prepare copper sulfate and ammonium iron sulfate solutions and test them to see if our reagent, potassium hexacynoferrate II will cause chemical changes that indicate that copper is present in one, and iron is present in the other.

I’m sure your brother will stay far away from your candy from now on. I know this is obvious, but don't eat anything in this lab or use it to coat candy... that was just an example to illustrate what an indicator is and how to use it.

Materials:
  • Erlenmeyer flask
  • Water
  • Potassium hexacynoferrate II K4Fe(CN)6 (MSDS)
  • Copper sulfate CuSO4 (MSDS)
  • Measuring spoon
  • Solid rubber stopper
  • Test tube rack
  • 3 Test tubes
  • Measuring syringe
  • Small labeled container for NaOH
  • Dropper pipette
  • Ammonium iron sulfate NH4Fe(SO4)2 (MSDS)
  • Sodium hydroxide NaOH (MSDS)
We need to remember to only make as much Potassium hexacynoferrate II as we need. Label test tubes with contents information clearly visible. Always remember that when the term “reagent” is used in chemistry it is referring to an indicator chemical.

We will put together two solutions with metallic chemicals dissolved in water in each. Let’s pretend we have no idea if they are metallic or not. Many times as a chemist, when analyzing a customer’s of a production chemical, we are looking for metal in a solution as an indicator of something good or something bad. If one of the factory’s systems contains a corrosive liquid flowing through its veins, it would be good to know if some metal somewhere is corroding, being dissolved, by the fluid. Our tests could confirm a problem or set the boss’s mind to rest….and get us a big bonus if we save the day.

C3000: Experiments

Here’s what’s going on in this experiment:
In test tube #1: Copper sulfate solution into which we added Potassium hexacynoferrate II. A color change occurred (brown) and a precipitate fell out of the liquid to rest on the bottom of the test tube. This reaction was an indicator for the presence of metal.

In test tube #2: Ammonium iron sulfate solution into which we added Potassium hexacynoferrate II. A color change occurred (dark blue). This reaction was an indicator for the presence of metal.

Cleanup: We are going to clean everything thoroughly after we finish the lab. After cleaning with soap and water, rinse thoroughly. Chemists use the rule of “three” in cleaning glassware and tools. After washing, chemists rinse out all visible soap and then rinse three times more.

Storage: Place cleaned tools and glassware in their respective storage places.

Disposal: Liquids, after neutralization, can be washed down the drain. Solids are thrown in the trash.

Click here to go to next lesson on Characteristics and capacity of buffers

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